Properties:
| RelatedCategories | Analytical/Chromatography,GELifeSciencesGelFiltrationColumns,GelFiltrationChromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations, | 
| material  | BedSupport(polyamide) | 
|   | borosilicateglasscolumn | 
|   | glassfiber(polypropylenereinforced) | 
| shelflife  | Pleasebeawarethisproductmaybeshipped90daysbeforetheexpirationdate.Formoreinformationonthebatchspecificexpirationdate,pleasecontacttechnicalservice. | 
| molwt  | molwt10000-600000 Da(globularproteins) | 
| packaging  | pkgof1 ea | 
| mfr.no.  | GEHealthcare,28-9893-35 | 
| parameter  | <1.0mL/minflowrate | 
|   | 0.3Mpa(42psi) | 
| bedvolume  | 120 mL | 
| columnL×I.D.  | 60 cm×16 mm | 
| samplevolume  | <5 mL | 
| matrix  | agarosedextrancomposite | 
|   | dextran-agaroseparticleplatform | 
| matrixactivegroup  | dextran-agarosephase | 
| avg.part.size  | 34 μm | 
| cleaning  | 1-14 | 
| workingrange  | 3-12 | 
| modeofchromatography  | Sizeexclusion(SEC) | 
Description:
AnalysisNote
ToviewtheCertificateofAnalysisforthisproduct,pleasevisitwww.geLifesciences.com
.
FeaturesandBenefits
• Highresolutionwithshortruntimesandgoodrecovery.
• Highcapacityathighflowrates.
• Separationscanbescaleduptoproductionlevels.
• Prepackedforconvenienceandreproducibility.
• 1/16”fittingsforconvenientconnectionstoAKTAdesignsystems.
Generaldescription
HiLoad®16/600Superdex®200prepgradeareprepackedXKcolumnsdesignedforhigh-resolutionpreparativegelfiltrationchromatography.ThecolumnisanexcellentchoiceforseparatingMAbsfromdimersandcontaminants.
Superdex®prepgrade(pg)isproducedbycovalentbondingofdextrantohighlycross-linkedagarose.Theseparationpropertiesofthesemediaaredeterminedbythedextrancomponent.Steepselectivitycurvesgiveexcellentresolvingpowerforpeptidesandproteinsinthemolecularweightranges,Mr<10000(Superdex®30pg),Mr3000to70000(Superdex®75pg),andMr10000600000(Superdex®200pg).Themediumcombineshighmechanicalstrengthwithhighhydrophilicity,allowinghighflowrates,andminimalnonspecificinteractions.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
SuitABIlity
Allcommonlyusedbuffers,1MNaOH,0.1MHCl,1Maceticacid,8Murea,6MguanidineHCl,1%SDS,70%ethanol,30%iso-propanol,30%acetonitrile
forhigh-resolutionpreparativegelfiltrationchromatography,e.g,separatemAbsfromdimersandcontaminants
LegalInformation
HiLoadisaregisteredtrademarkofGEHealthcare
SuperdexisaregisteredtrademarkofGEHealthcare
Safety:
RIDADR 
NONHforallmodesoftransport